ZYMV is a common virus
infecting wax gourd in Guangdong Province, but there are few systematic
researches about this virus. In this research, researchers detected the
infection situation and genetic variation of coat protein gene of ZYMV isolates
in Guangdong Province, and lays a foundation for further pathogenicity research
and antivirus genetic engineering.
Source: Baidu
Zucchini
yellow mosaic virus (ZYMV) is a common virus infecting wax gourd in Guangdong Province,
but there are few systematic researches about this virus. In this research,
researchers detected the infection situation and genetic variation of coat
protein gene of ZYMV isolates in Guangdong Province, and lays a foundation for
further pathogenicity research and antivirus genetic engineering.
Researcher collected and detected 76 wax gourd samples from 9 main production
regions in Guangdong Province based on RT-PCR amplification of coat protein
gene (CP) of ZYMV with RNA extraction methods of wax gourd leaves and carried
out genetic variation analysis with sequenced amplification products.
The results show that 24 samples are determined as positive with incidence
ratio of 31.59%, and sequence analysis reveals that full-length of ZYMV-CP of
16 isolates is 840 bp encoding a peptide with 279 amino acids. Further
alignment analysis indicates that similarities of ZYMV-CP nucleotide acid and
amino acid sequences range from 98.10% to 100% and from 96.10% to 100%,
respectively. The motifs of deduced amino acids sequences are conserved, all
isolates contain 5 Human Leukocyte Antigen (HLA) sites and 1 N-glycosylation
site expects GD121-9 with 2 additive HLA motifs. The CP protein of all isolates
includes sconserved domain potyv_CP and DAG triple box (Asp-Ala-Gly) crucial
for potyvirus transmission by aphid. Phylogenetic analysis suggests that all
isolates are clustered into 7 genotypes.
Guangdong isolates belong to genotype I that contains three strains. All
Guangdong isolates are further clustered into three groups with no obvious
geographical differentiation. Generally, ZYMV-CP in Guangdong isolates is
conserved and has little molecular variation.
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